UPRAWA / ROZMNAŻANIE / 3

Asymbiotic micropropagation of orchids from seeds (flasking)

16.
The earliest stage of orchid "seedling" development is known as a protocorm.

Question: How does the orchid protocorm differ from the earliest stage of seedling development in other (non orchid) higher plant species?

Continue to observe and sketch the subsequent stages of orchid seedling development.

Question: Approximately how old is the seedling when the first root emerges from the base of the 1st leaf. Is this 1st root adventitious or not?

17.
Subculture: When seedlings are about the size shown in this plate...

On the day of the class lab you will be given a plate of pregerminated seedlings at the appropriate stage of development for you to subculture.

18.
...they should be subcultured to a fresh plate of the same medium at a spacing of approx. 15 mm between seedlings.